Membrane Transporter

SURFE²R N1

The technology employs solid supported membrane (SSM) -based electrophysiology which was established in the late 1990s. The reusable sensors contain a gold-coating on which the SSM is formed in a quick pipetting process. Then the sample containing your transporter of interest is added on top to physically adsorb to the SSM. Any kind of membrane preparation containing the protein of interest can be used for measurements, e.g. membrane vesicles after cell disruption or proteoliposomes after reconstitution of purified proteins. These samples can be stored frozen for months and years. Therefore no running cell culture lab is required.
The key to SSM-based electrophysiology is the exchange of solutions to provide the substrate or ligand and activate the transporter. The following charge translocation is detected and can be analyzed. Due to the high stability of the SSM up to one hundred sequential measurements can be performed on the same sample. This allows for determination of parameters such as EC50 or IC50. Since the time resolution of solution exchange is state-of-the-art, not only slow transport can be measured, but also fast binding reactions can be assayed and rate constants can be determined.

SURFE²R 96SE

Just like the SURFE2R N1, the SURFE2R 96SE employs solid supported membrane (SSM)-based electrophysiology to resolve small currents of transporters and pumps but elevates the throughput to the next level by parallelization of 96 measurements. The transporter samples are added to gold-coated sensors in a standard 96 well-plate format. To further reduce the user's workload, most sensor coating steps can be done by the SURFE2R 96SE itself. Any substrate can be added to activate the transporter. The resulting transport or binding currents will be detected and analyzed accordingly. The process from sensor preparation and measurement to data analysis is fully automated.
The SURFE2R 96SE is preferred over the SURFE2R N1 when a high throughput environment is needed. This is the case, e.g. for compound or transporter screening projects. The SURFE2R 96SE is also suitable to increase throughput for basic research. Data generation is approximately 100 times faster compared to the SURFE2R N1.

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